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KMID : 1134820110400010014
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Journal of the Korean Society of Food Science and Nutrition
2011 Volume.40 No. 1 p.14 ~ p.19
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Screen of Functional Activity of Polysaccharide and Glycosaminoglycan from Sea Hare ( Aplysia kurodai) by Cell Line
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Hong Yu-Mi
Park Si-Hyang
Yoon Bo-Yeong
Choi Byeong-Dai
Choi Yeung-Joon
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Abstract
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In this study, we extracted a whole polysaccharide fraction from the sea hare, Aplysia kurodai, and screened its functional properties using cell lines. The functionalities of polysaccharide and glycosaminoglycan (GAG) were investigated with RAW 264.7 cell lines. The crude polysaccharides and GAG purified DEAE-Sepharose chromatography did not show the toxicity on RAW 264.7 cell line in the range of 10～200 ㎍/mL, whereas they increased the cell growth rate. The crude polysaccharides and purified GAG also increased the production of NO, interleukin-6 and tumor necrosis factor-α on RAW 264.7 cell. Particularly, the purified GAG inhibited the proliferation of stomach cancer cell line, AGS, up to 40% for 72 hr incubation, but not the intestinal epithelial IEC-6 cell lines.
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KEYWORD
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sea hare, Aplysia kurodai, glycosaminoglycan, functional activity
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